oral epithelial cell line hok  (Procell Inc)

Journal: OncoTargets and therapy

Article Title: LINC00958-MYC positive feedback loop modulates resistance of head and neck squamous cell carcinoma cells to chemo- and radiotherapy in vitro

doi: 10.2147/OTT.S208318

Figure Lengend Snippet: Induction of HNSCC cell sensitivity to ionizing radiation and cisplatin after knockdown of LINC00958 expression. ( A ) qRT-PCR. The relative expression of LINC00958 was assessed in HNSCC cell lines (Detroit 562, Cal27, SCC-9, SCC-15, and FaDu) and a normal oral epithelial cell line (HOK). ( B ) qRT-PCR. LINC00958 expression was manipulated using LINC00958 siRNAs or LINC00958 cDNA in Fadu and Detroit562 cells, respectively and assessed by using qRT-PCR. ( C ) CCK-8 assay. LINC00958 expression was manipulated using LINC00958 siRNAs or LINC00958 cDNA in Fadu and Detroit562 cells, respectively and assessed for changed cell viability using the CCK-8 assay. ( D and F ) CCK-8 assay. Fadu cells were grown and transfected with LINC00958 siRNAs and then subjected to different doses of ionizing radiation (0-10Gy) ( D ) or cisplatin (0–25 μM) ( F ) and the CCK-8 assay. ( E and G ) Colony formation assay. Fadu cells were grown and transfected with LINC00958 siRNAs and then subjected to 2 Gy ionizing radiation ( E ) or 5 µM cisplatin ( G ) and were subjected to colony formation assay. The data are the means ± SD (n=3). * p <0.05 and ** p <0.01 by two-tailed Student’s t -test.

Article Snippet: Human HNSCC cell lines (Detroit 562, Cal27, SCC-9, SCC-15, and Fadu) and a normal oral epithelial cell line (HOK) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, CCK-8 Assay, Transfection, Colony Assay, Two Tailed Test